Scot Fleming and Liverpool's Skupski broke into the world's top 50 this summer after reaching the final in Eastbourne but 2010 has proved challenging and they have now decided to go their separate ways.
The news is a blow for Great Britain Davis Cup captain Leon Smith, who considered having a pair who play together on the main tour a major asset.
Fleming told the LTA website: "Ken and I are very proud of our achievements as a team. We came through Futures and then Challengers to win two ATP titles, play all four grand slams and represent Great Britain in Davis Cup.
"We will always be good friends but have decided that on the court it will be best for both of us to freshen things up with new partners as we look to reach the next level in tennis."
Fleming and Skupski will play their final tournament together in defence of their title in St Petersburg next week.
Courtesy: Daily Express